July 29, 2019 at 1:34 pm

Chen Publishes ‘Photobleaching of YOYO-1 in Super-Resolution Single DNA Fluorescence Imaging’

Dr. Jixin Chen in white lab coat

Dr. Jixin Chen

Dr. Jixin Chen, Professor of Chemistry & Biochemistry, co-authored a paper on “Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging.”

Chen is also a member of the Nanoscale & Quantum Phenomena Institute at Ohio University.

Authors: Pyle, JR; Chen, JX

Source: BEILSTEIN JOURNAL OF NANOTECHNOLOGY, Volume 9, Issue 74, p.809-811; DOI:10.3762/bjnano.9.74

Published: March 2018

Abstract: Super-resolution imaging of single DNA molecules via point accumulation for imaging in nanoscale topography (PAINT) has great potential to visualize fine DNA structures with nanometer resolution. In a typical PAINT video acquisition, dye molecules (YOYO-1) in solution sparsely bind to the target surfaces (DNA) whose locations can be mathematically determined by fitting their fluorescent point spread function. Many YOYO-1 molecules intercalate into DNA and remain there during imaging, and most of them have to be temporarily or permanently fluorescently bleached, often stochastically, to allow for the visualization of a few fluorescent events per DNA per frame of the video. Thus, controlling the fluorescence on–off rate is important in PAINT. In this paper, we study the photobleaching of YOYO-1 and its correlation with the quality of the PAINT images. At a low excitation laser power density, the photobleaching of YOYO-1 is too slow and a minimum required power density was identified, which can be theoretically predicted with the proposed method in this report.

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