January 2, 2020 at 6:15 pm

Physics Colloquium | Resolving Single Molecules beyond the Diffraction Limit of Light, Jan. 24

The Physics & Astronomy Colloquium Series presents Jixin Chen of Department of Chemistry and Biochemistry, Ohio University on “Resolving Single Molecules beyond the Diffraction Limit of Light”, on Friday, Jan. 24, at 4:10 p.m. in Clippinger Labs 194.

Photo of Jixin Chen

Jixin Chen

Abstract: Super-resolution optical microscopy/nanoscopy is a technique that has been widely applied in many research fields recently, such as single-cell imaging, single-particle catalysis, single-molecule/particle diffusion, and single-molecule reactions. All traditional optical microscopes and telescopes have a theoretical spatial resolution at about half the wavelength of light defined by their point-spread functions (e.g. ~300 nm for visible lights). Super-resolution microscopy breaks the limit with two methods to sub-100 nm even sub-1 nm resolution regions: (1) structuring and combining multiple point-spread functions, and (2) single-molecule localization.

In this colloquium, Dr. Chen will briefly introduce the history of this technique and then talk about the applications of the second method in his group. He will focus on the topic of resolving single-DNA molecules using dye-labeling, as well as resolving photochemical reactions at the single-molecule level, namely, the generation of reactive oxygen species upon shining light to dye-labeled DNA molecules. If time allowed, he will also briefly introduce the applications of the technique in photophysical measurements of single lead halide nanoparticles.

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